5AF4
Structure of Lys33-linked diUb
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 110 |
Detector technology | PIXEL |
Collection date | 2014-02-14 |
Detector | PILATUS |
Spacegroup name | I 4 |
Unit cell lengths | 113.075, 113.075, 103.899 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.469 - 1.850 |
R-factor | 0.2326 |
Rwork | 0.231 |
R-free | 0.25630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ubq |
RMSD bond length | 0.005 |
RMSD bond angle | 0.928 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.470 | 1.890 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.040 | 0.440 |
Number of reflections | 55562 | |
<I/σ(I)> | 11.9 | 2.5 |
Completeness [%] | 99.8 | 100 |
Redundancy | 3.5 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 3M AMMONIUM SULPHATE, 100 MM TRIS PH 8.0 |