5ADW
The Periplasmic Binding Protein CeuE of Campylobacter jejuni preferentially binds the iron(III) complex of the Linear Dimer Component of Enterobactin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-11 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 1 |
| Unit cell lengths | 58.461, 63.021, 67.011 |
| Unit cell angles | 83.36, 76.57, 78.24 |
Refinement procedure
| Resolution | 65.010 - 1.900 |
| R-factor | 0.22676 |
| Rwork | 0.225 |
| R-free | 0.26407 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zkw |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.775 |
| Data reduction software | xia2 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.760 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.100 | 0.900 |
| Number of reflections | 70279 | |
| <I/σ(I)> | 17.5 | 2.2 |
| Completeness [%] | 98.1 | 96.1 |
| Redundancy | 4.4 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.1 M MMT (DL-MALIC ACID, MES, TRIS, 1:2:2) BUFFER, PH 8.0, 25% (W/V) PEG 1500 |
