5ABV
Complex of D. melanogaster eIF4E with the 4E-binding protein Mextli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-10-06 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 65.900, 82.020, 84.730 |
Unit cell angles | 90.00, 90.13, 90.00 |
Refinement procedure
Resolution | 43.960 - 2.130 |
R-factor | 0.1937 |
Rwork | 0.192 |
R-free | 0.23180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4UE8 CHAIN A |
RMSD bond length | 0.010 |
RMSD bond angle | 1.050 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.000 | 2.190 |
High resolution limit [Å] | 2.130 | 2.130 |
Number of reflections | 50587 | |
<I/σ(I)> | 13 | 2 |
Completeness [%] | 98.2 | 90.5 |
Redundancy | 4.5 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 0.1M BISTRIS PH=6.0, 23% PEG3350 |