5A6J
GH20C, Beta-hexosaminidase from Streptococcus pneumoniae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-002 |
Temperature [K] | 291 |
Detector technology | CCD |
Detector | RIGAKU R-AXIS IV++ |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 92.840, 136.940, 202.350 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 113.410 - 1.940 |
R-factor | 0.20853 |
Rwork | 0.206 |
R-free | 0.25790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2epn |
RMSD bond length | 0.009 |
RMSD bond angle | 1.283 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.700 | 2.050 |
High resolution limit [Å] | 1.940 | 1.940 |
Rmerge | 0.110 | 0.340 |
Number of reflections | 184285 | |
<I/σ(I)> | 9.2 | 3.9 |
Completeness [%] | 97.4 | 96.9 |
Redundancy | 3.8 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 10.2 | 12% (W/V) PEG 3350, 0.2 M MAGNESIUM CHLORIDE, 0.05 M CAPS, PH 10.2, 1% GLYCEROL |