5A61
Crystal structure of full-length E. coli ygiF in complex with tripolyphosphate and two manganese ions.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-05-15 |
Detector | DECTRIS PILATUS |
Wavelength(s) | 0.999990, 1.700020 |
Spacegroup name | P 65 |
Unit cell lengths | 89.676, 89.676, 125.329 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.770 - 1.500 |
R-factor | 0.15242 |
Rwork | 0.151 |
R-free | 0.17087 |
Structure solution method | OTHER |
Starting model (for MR) | NONE |
RMSD bond length | 0.006 |
RMSD bond angle | 1.186 |
Data reduction software | XDS |
Data scaling software | XDS |
Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.770 | 1.590 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.040 | 1.340 |
Number of reflections | 91340 | |
<I/σ(I)> | 26.35 | 2.03 |
Completeness [%] | 99.8 | 99.1 |
Redundancy | 15.48 | 14.18 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 20% PEG 3,350, 0.2 M NACL |