4ZTT
Crystal structures of ferritin mutants reveal diferric-peroxo intermediates
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 298.15 |
Detector technology | CCD |
Collection date | 2013-11-15 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.98 |
Spacegroup name | I 4 |
Unit cell lengths | 128.816, 128.816, 172.247 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.660 - 1.830 |
R-factor | 0.15813 |
Rwork | 0.156 |
R-free | 0.19191 |
RMSD bond length | 0.027 |
RMSD bond angle | 2.061 |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 1.600 |
Number of reflections | 154787 |
<I/σ(I)> | 17 |
Completeness [%] | 91.6 |
Redundancy | 7.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298.15 | Lithium sulfate monohydrate, sodium citrate, ammonium sulfate |