4ZS3
Structural complex of 5-aminofluorescein bound to the FTO protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-01-12 |
Detector | APEX II CCD |
Wavelength(s) | 0.9735 |
Spacegroup name | H 3 |
Unit cell lengths | 142.239, 142.239, 83.426 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.910 - 2.450 |
R-factor | 0.20889 |
Rwork | 0.206 |
R-free | 0.26257 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.160 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.450 |
Number of reflections | 23094 |
<I/σ(I)> | 27.6 |
Completeness [%] | 100.0 |
Redundancy | 10.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 100 mM sodium citrate, 11% PEG3350, 10% isopropanol |