4ZRI

Crystal structure of Merlin-FERM and Lats2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9791
Spacegroup name	P 1 21 1
Unit cell lengths	62.962, 100.549, 65.030
Unit cell angles	90.00, 105.85, 90.00

Refinement procedure

Resolution	36.008 - 2.700
R-factor	0.2289
Rwork	0.227
R-free	0.27040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1isn
RMSD bond length	0.010
RMSD bond angle	1.256
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.790
High resolution limit [Å]	2.700	2.700
Rmerge		0.406
Number of reflections	21376
<I/σ(I)>	20.4	2.2
Completeness [%]	99.3	95
Redundancy	3.8	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	18% iso-Propanol, 0.1M Tris, 5% PEG8000