4ZMM
GGDEF domain of Dcsbis complexed with c-di-GMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-01-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 93.275, 42.707, 106.372 |
| Unit cell angles | 90.00, 106.75, 90.00 |
Refinement procedure
| Resolution | 37.618 - 2.503 |
| R-factor | 0.2038 |
| Rwork | 0.201 |
| R-free | 0.24770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Full-length Dcsbis |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.317 |
| Data reduction software | SCALEPACK |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 13825 | |
| <I/σ(I)> | 46.34 | |
| Completeness [%] | 98.2 | 99.4 |
| Redundancy | 3.4 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293.15 | 0.5 M sodium citrate tribasic dehydrate, 0.1 M Tris-HCl pH 8.5 |
