4ZDO
The crystal structure of T325S mutant of human SepSecS in complex with selenocysteine tRNA (tRNASec)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2014-02-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 31 1 2 |
| Unit cell lengths | 166.238, 166.238, 238.160 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.492 - 2.650 |
| R-factor | 0.1996 |
| Rwork | 0.198 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hl2 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.044 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 6.510 | 2.400 |
| Rmerge | 0.152 | 0.060 | |
| Rmeas | 0.161 | 0.063 | |
| Rpim | 0.059 | 0.019 | |
| Total number of observations | 1085896 | ||
| Number of reflections | 133611 | ||
| <I/σ(I)> | 5 | ||
| Completeness [%] | 90.9 | 99.9 | 35 |
| Redundancy | 8.1 | 11 | 1.5 |
| CC(1/2) | 0.995 | 0.030 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 285 | 0.1M Na-cacodylate, pH 6.4 0.24M lithium citrate 10-12% (w/v) PEG 3,350 |
