4ZBM
Crystal structure of Drosophila cyclic nucleotide gated channel pore mimicking NaK mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 78 |
Detector technology | CCD |
Collection date | 2008-02-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | I 4 |
Unit cell lengths | 67.907, 67.907, 89.799 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.900 |
Rwork | 0.204 |
R-free | 0.23610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3k0d |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 15224 | |
<I/σ(I)> | 19 | 0.97 |
Completeness [%] | 95.0 | |
Redundancy | 6.5 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 293 | 65%MPD, 100mM KCl, 100mM MES, 4mM n-Decyl-beta-D-Maltoside |