4Z7Y
diphosphomevalonate decarboxylase from the Sulfolobus solfataricus, space group P21
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2013-01-26 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 94.900, 154.390, 109.969 |
Unit cell angles | 90.00, 114.41, 90.00 |
Refinement procedure
Resolution | 47.630 - 2.700 |
R-factor | 0.25535 |
Rwork | 0.254 |
R-free | 0.27663 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hk2 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.496 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | BALBES |
Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.700 |
Number of reflections | 78276 |
<I/σ(I)> | 16.4 |
Completeness [%] | 96.6 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 0.1M sodium acetate, 1.5M ammonium sulfate |