4Z7K
Crystal structure of CRISPR RNA processing endoribonuclease Cas6b
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-07-08 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.000 |
Spacegroup name | P 31 |
Unit cell lengths | 92.122, 92.122, 96.790 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.592 - 3.000 |
R-factor | 0.2294 |
Rwork | 0.224 |
R-free | 0.27840 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.717 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.900 |
Number of reflections | 16169 |
<I/σ(I)> | 36.7 |
Completeness [%] | 83.6 |
Redundancy | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 303 | 100 mM KCl, 10mM MgCl2, 50mM sodium cacodylate pH 6.5, 12% PEG4000. |