4YM0
Crystal structure of the human galectin-4 C-terminal carbohydrate recognition domain in complex with lacto-N-tetraose (LNT)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-15 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.9537 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.189, 130.670, 45.271 |
Unit cell angles | 90.00, 96.35, 90.00 |
Refinement procedure
Resolution | 42.540 - 2.300 |
R-factor | 0.1738 |
Rwork | 0.172 |
R-free | 0.20790 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.366 |
Data reduction software | XDS |
Data scaling software | Aimless (0.3.8) |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 44.990 | 44.990 | 2.380 |
High resolution limit [Å] | 2.300 | 8.910 | 2.300 |
Rmerge | 0.028 | 0.018 | 0.106 |
Rpim | 0.016 | 0.010 | 0.071 |
Total number of observations | 93135 | 1519 | 6257 |
Number of reflections | 22849 | ||
<I/σ(I)> | 35.2 | 65.6 | 10 |
Completeness [%] | 98.6 | 96.6 | 88.8 |
Redundancy | 4.1 | 3.8 | 3.1 |
CC(1/2) | 0.999 | 0.999 | 0.978 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.15 | 1.2 M ammonium sulfate, 0.1 M HEPES pH 7.0, 4 % v/v PEG 400 |