4YJS

THE KINASE DOMAIN OF HUMAN SPLEEN TYROSINE (SYK) IN COMPLEX WITH GTC000226

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	OTHER
Temperature [K]	100
Detector technology	CCD
Collection date	2005-03-03
Detector	MAR CCD 130 mm
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	38.407, 84.617, 39.756
Unit cell angles	90.00, 100.38, 90.00

Refinement procedure

Resolution	20.000 - 2.220
R-factor	0.1722
Rwork	0.170
R-free	0.22570
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	In-house SYK model
RMSD bond length	0.011
RMSD bond angle	1.444
Data reduction software	MOSFLM (6.2.4)
Data scaling software	SCALA (3.2.5)
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.712	39.103	2.340
High resolution limit [Å]	2.220	7.020	2.220
Rmerge	0.059	0.027	0.486
Total number of observations	45294	1479	6459
Number of reflections	12409
<I/σ(I)>	18.7	59.5	2.3
Completeness [%]	100.0	98.7	100
Redundancy	3.7	3.6	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	293	32.5% Jeffamine ED-2001, 10% Glycerol, 0.1M MES pH6.8, 5mM DTT