4YG0
Structural basis of glycan recognition in neonate-specific rotaviruses
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-14 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.977408 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 32.210, 64.050, 70.920 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.020 - 1.285 |
R-factor | 0.1721 |
Rwork | 0.171 |
R-free | 0.19940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4yfw |
RMSD bond length | 0.007 |
RMSD bond angle | 1.102 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.020 | 1.350 |
High resolution limit [Å] | 1.280 | 1.280 |
Rmerge | 0.065 | 0.283 |
Number of reflections | 35249 | |
<I/σ(I)> | 17.1 | 6.9 |
Completeness [%] | 92.2 | 99.8 |
Redundancy | 8.1 | 7.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.2M NaCl, 0.1M Imidazole, 1.0M Potasium/Sodium tartrate |