Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-11-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 171.370, 538.660, 310.920 |
Unit cell angles | 90.00, 104.09, 90.00 |
Refinement procedure
Resolution | 49.099 - 3.000 |
R-factor | 0.2753 |
Rwork | 0.274 |
R-free | 0.30310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3j32 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.402 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 49.100 |
High resolution limit [Å] | 3.000 |
Number of reflections | 1072359 |
<I/σ(I)> | 7.76 |
Completeness [%] | 98.9 |
Redundancy | 2.56 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG400, HEPES, CaCl2 |