4Y2F

CRYSTAL STRUCTURE OF NATIVE GAF DOMAIN of POTASSIUM SENSOR HISTIDINE KINASE KDPD FROM ESCHERICHIA COLI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-10-09
Detector	RIGAKU RAXIS IV++
Wavelength(s)	0.97932
Spacegroup name	P 21 21 2
Unit cell lengths	35.250, 57.540, 61.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.058 - 1.396
R-factor	0.183
Rwork	0.181
R-free	0.20480
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.886
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((dev-2313_1839: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.770	1.446
High resolution limit [Å]	1.390	1.396
Number of reflections	24876
<I/σ(I)>	18
Completeness [%]	98.3	97.1
Redundancy	14.1	13.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	30-35% PEG 3350, 0.1 M Bis-TRIS pH 6.0 - 6.5