4XXW
Crystal structure of mouse Cadherin-23 EC1-2 and Protocadherin-15 EC1-2 splice variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-31 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97920 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 157.746, 57.789, 155.264 |
| Unit cell angles | 90.00, 99.17, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.261 |
| R-factor | 0.18945 |
| Rwork | 0.187 |
| R-free | 0.23288 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4apx |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.806 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.300 |
| High resolution limit [Å] | 2.261 | 6.130 | 2.261 |
| Rmerge | 0.079 | 0.043 | 0.402 |
| Rmeas | 0.094 | 0.051 | 0.494 |
| Rpim | 0.050 | 0.026 | 0.283 |
| Total number of observations | 200718 | ||
| Number of reflections | 62947 | ||
| <I/σ(I)> | 6.7 | 2.3 | |
| Completeness [%] | 96.8 | 98.7 | 84.9 |
| Redundancy | 3.2 | 3.6 | 2.5 |
| CC(1/2) | 0.994 | 0.844 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.2 M Ammonium Acetate 0.1 M HEPES, pH 7.5 25% (v/v) isopropanol |
