4XNO
Crystal structure of 5'-CTTATPPPZZZATAAG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2014-07-24 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.91963 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 42.019, 42.019, 140.472 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.227 - 1.985 |
R-factor | 0.2137 |
Rwork | 0.212 |
R-free | 0.23760 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.829 |
Data scaling software | SCALEPACK |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 100.000 | 2.010 |
High resolution limit [Å] | 1.980 | 5.370 | 1.980 |
Rmerge | 0.069 | 0.041 | 0.768 |
Rmeas | 0.072 | 0.043 | 0.843 |
Rpim | 0.022 | 0.013 | 0.339 |
Total number of observations | 105588 | ||
Number of reflections | 10571 | ||
<I/σ(I)> | 20 | ||
Completeness [%] | 99.5 | 92.5 | 98.8 |
Redundancy | 10 | 9.4 | 5.6 |
CC(1/2) | 0.998 | 0.728 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | Ammonium sulfate |