4XNK
X-ray structure of AlgE1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2014-05-01 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.033 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.010, 74.340, 184.690 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.420 - 2.800 |
R-factor | 0.2291 |
Rwork | 0.226 |
R-free | 0.27380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4afk |
RMSD bond length | 0.008 |
RMSD bond angle | 1.070 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 16066 | |
<I/σ(I)> | 4 | 1.1 |
Completeness [%] | 94.3 | 96 |
Redundancy | 5.6 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293 | 450 mM NH4Cl, 21 %(v/v) PEG 400, 100 mM MES buffer pH 6.5 |