4XN7

Crystal Structure of E. coli Aminopeptidase N in complex with L-2,3-Diaminopropionic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-03-02
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54179
Spacegroup name	P 31 2 1
Unit cell lengths	120.690, 120.690, 170.460
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.250 - 2.220
R-factor	0.2013
Rwork	0.199
R-free	0.24589
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hpo
RMSD bond length	0.013
RMSD bond angle	1.648
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.250	2.350
High resolution limit [Å]	2.220	2.220
Number of reflections	70135
<I/σ(I)>	5.31	2.78
Completeness [%]	99.5	99
Redundancy	5	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	Sodium malonate