4XB3
Structure of dextran glucosidase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-12-11 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9800 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 72.151, 82.550, 103.559 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.108 - 2.093 |
R-factor | 0.1813 |
Rwork | 0.179 |
R-free | 0.21900 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.704 |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.220 |
High resolution limit [Å] | 2.090 | 2.090 |
Number of reflections | 36936 | |
<I/σ(I)> | 14.81 | 4.45 |
Completeness [%] | 99.4 | 97.4 |
Redundancy | 5.84 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 100mM Sodium HEPES, 200mM CaCl2, 180mg/ml PEG6000 |