4WPA
Crystal structure of Adenylyl cyclase Ma1120 from Mycobacterium Avium bound to Pyrophosphate and Calcium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-29 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.720, 55.720, 55.330 |
Unit cell angles | 90.00, 110.22, 90.00 |
Refinement procedure
Resolution | 37.985 - 1.700 |
R-factor | 0.1897 |
Rwork | 0.188 |
R-free | 0.22440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4wp8 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.054 |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.470 | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.068 | 0.636 |
Number of reflections | 33241 | |
<I/σ(I)> | 10.4 | 2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 3.8 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 7.5 | 291 | 0.1M HEPES, 30%(w/v) PEG 1000 |