4WNG
Crystal structure of the TPR domain of LGN in complex with Frmpd4/Preso1 at 2.1 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2014-02-10 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.1000 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 90.527, 90.527, 177.067 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.580 - 2.110 |
| R-factor | 0.2268 |
| Rwork | 0.224 |
| R-free | 0.27120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wne |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.066 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP (11.0.05) |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 5.700 | 2.100 |
| Rmerge | 0.093 | 0.040 | 0.951 |
| Rmeas | 0.096 | 0.041 | 0.985 |
| Rpim | 0.023 | 0.010 | 0.257 |
| Total number of observations | 442380 | ||
| Number of reflections | 25343 | ||
| <I/σ(I)> | 8.2 | 2.308 | |
| Completeness [%] | 99.9 | 99.2 | 100 |
| Redundancy | 17.5 | 17.1 | 14.4 |
| CC(1/2) | 0.999 | 0.915 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1 M BisTrispropane (pH 8.0), 0.2 M magnesium chloride, 20% PEG 3350 |
