4WN4
Crystal structure of designed cPPR-polyA protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-06-13 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.07253 |
Spacegroup name | F 2 3 |
Unit cell lengths | 204.720, 204.720, 204.720 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.791 - 3.850 |
R-factor | 0.1836 |
Rwork | 0.179 |
R-free | 0.23040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4pjq |
RMSD bond length | 0.011 |
RMSD bond angle | 1.361 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.950 |
High resolution limit [Å] | 3.850 | 3.850 |
Rmerge | 0.100 | 1.960 |
Number of reflections | 6865 | |
<I/σ(I)> | 23 | 2.18 |
Completeness [%] | 99.9 | 100 |
Redundancy | 20 | 19.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.2 M magnesium chloride, 0.1 M Tris pH 7.0, 10% w/v PEG 8000 |