4WJN
Crystal structure of SUMO1 in complex with phosphorylated PML
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-09-14 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.274, 47.079, 63.986 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.921 - 1.500 |
R-factor | 0.16 |
Rwork | 0.158 |
R-free | 0.19210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2uyz |
RMSD bond length | 0.010 |
RMSD bond angle | 1.298 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: dev_1555)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.921 | 1.580 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.112 | 0.870 |
Number of reflections | 18507 | |
<I/σ(I)> | 11.5 | 2.1 |
Completeness [%] | 96.7 | 81.8 |
Redundancy | 9.8 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 100mM sodium cacodylate, 16% PEG3350, 10mM calcium chloride |