4WD3
Crystal structure of an L-amino acid ligase RizA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-03-05 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9788 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.380, 87.290, 85.260 |
Unit cell angles | 90.00, 97.52, 90.00 |
Refinement procedure
Resolution | 48.238 - 2.800 |
R-factor | 0.2042 |
Rwork | 0.201 |
R-free | 0.25480 |
Structure solution method | SAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.678 |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.240 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.091 | 0.294 |
Number of reflections | 20941 | |
<I/σ(I)> | 15.5 | 7 |
Completeness [%] | 99.5 | 99.7 |
Redundancy | 7.3 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | sodium HEPES, magnesium chloride, polyethylene glycol 3350, sodium iodide |