4WAE

Phosphatidylinositol 4-kinase III beta crystallized with ATP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-08-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.918409
Spacegroup name	P 43
Unit cell lengths	108.327, 108.327, 55.189
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.445 - 3.318
R-factor	0.1855
Rwork	0.182
R-free	0.24410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4d0m
RMSD bond length	0.012
RMSD bond angle	1.584
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	48.450
High resolution limit [Å]	3.318
Number of reflections	9684
<I/σ(I)>	6.59
Completeness [%]	99.8
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	10% w/v PEG 4000, 20% v/v glycerol 0.1 M MOPS/HEPES-Na pH 7.5