4W8Q
Crystal structure of truncated hemolysin A from P. mirabilis at 1.4 Angstroms resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-07-03 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1.03 |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 59.719, 34.317, 68.309 |
Unit cell angles | 90.00, 99.21, 90.00 |
Refinement procedure
Resolution | 29.658 - 1.428 |
R-factor | 0.1401 |
Rwork | 0.139 |
R-free | 0.16290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3fy3 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.523 |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.700 | 1.460 |
High resolution limit [Å] | 1.428 | 1.428 |
Rmerge | 0.078 | 0.814 |
Number of reflections | 49625 | |
<I/σ(I)> | 25.4 | 2.2 |
Completeness [%] | 97.2 | 74.9 |
Redundancy | 6.9 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 100 mM citrate pH 5.5, 100 mM NaCl, PEG 4000 (8 - 16%) |