4W7A
Crystal Structure of Full-Length Split GFP Mutant D21H/K26C Disulfide and Metal-Mediated Dimer, P 21 21 21 Space Group, Form 4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-10-31 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 120.830, 121.330, 192.560 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 96.280 - 3.603 |
R-factor | 0.2804 |
Rwork | 0.278 |
R-free | 0.30180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2b3p |
RMSD bond length | 0.011 |
RMSD bond angle | 1.220 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 96.280 |
High resolution limit [Å] | 3.603 |
Number of reflections | 33244 |
<I/σ(I)> | 13.95 |
Completeness [%] | 99.4 |
Redundancy | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 3M NaCl, 0.1M Bis-Tris pH 5.5 |