4W1Y
Crystal structure of Escherichia coli Tryptophanase in 'semi-holo' form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-08-01 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 109.971, 109.971, 238.403 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 3.200 |
R-factor | 0.21772 |
Rwork | 0.214 |
R-free | 0.28972 |
RMSD bond length | 0.022 |
RMSD bond angle | 2.385 |
Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 3.300 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.519 | |
Number of reflections | 22389 | |
<I/σ(I)> | 19.2 | 3.1 |
Completeness [%] | 94.8 | 97.2 |
Redundancy | 5.9 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 13-16 % AMMONIUM SULFATE, 1-5 MM PLP, 50 MM TRIS |