4V9O
Control of ribosomal subunit rotation by elongation factor G
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-12-03 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 361.600, 361.770, 433.200 |
Unit cell angles | 90.00, 103.57, 90.00 |
Refinement procedure
Resolution | 70.000 - 2.900 |
Rwork | 0.223 |
R-free | 0.27150 |
Structure solution method | MOLECULAR REPLACEMENT |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 70.000 |
High resolution limit [Å] | 2.900 |
Number of reflections | 1874109 |
Completeness [%] | 83.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 6.5 | 291 | PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K |
1 | MICROBATCH | 6.5 | 291 | PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K |