4V9K
70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state.
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-06-07 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.03322 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 306.009, 673.491, 351.984 |
Unit cell angles | 90.00, 92.69, 90.00 |
Refinement procedure
Resolution | 40.000 - 3.500 |
R-factor | 0.262 |
Rwork | 0.262 |
R-free | 0.30900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB Entries 3F1E 3F1F 2WRI 2WRJ |
RMSD bond length | 0.042 |
RMSD bond angle | 1.070 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 351.598 |
High resolution limit [Å] | 3.497 |
Number of reflections | 2605789 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM | |||
1 | 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM |