4V74
70S-fMetVal-tRNAVal-tRNAfMet complex in hybrid pre-translocation state (pre5b)
This is a non-PDB format compatible entry.
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
| Method | Manual blotting for about 2 seconds |
EM Data Collection
| Microscope | FEI/PHILIPS CM200FEG |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 160 |
| Cs (mm) | 2 |
| Detector | GENERIC TVIPS (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 17 |
| Software | DireX,Custom,IMAGIC,SPIDER |
| Symmetry | C1 |
