4V3E
The CIDRa domain from IT4var07 PfEMP1 bound to endothelial protein C receptor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-12-12 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 56.019, 56.019, 250.505 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 83.500 - 2.900 |
R-factor | 0.2487 |
Rwork | 0.247 |
R-free | 0.27610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1l8j |
RMSD bond length | 0.011 |
RMSD bond angle | 1.260 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 83.900 | 3.060 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.060 | 0.880 |
Number of reflections | 10832 | |
<I/σ(I)> | 16.4 | 2.3 |
Completeness [%] | 99.9 | 99.1 |
Redundancy | 8.4 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 0.2 M NANO3, 0.1 M BTP PH 7.5, 20% PEG 3350 |