4V0T
Monomeric pseudorabies virus protease pUL26N at 2.1 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-30 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.168, 98.611, 131.109 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 78.810 - 2.050 |
| R-factor | 0.18122 |
| Rwork | 0.179 |
| R-free | 0.22704 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4v07 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.993 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.800 | 2.160 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.070 | 0.800 |
| Number of reflections | 33525 | |
| <I/σ(I)> | 16.33 | 2.69 |
| Completeness [%] | 99.6 | 97.8 |
| Redundancy | 6.5 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.1 M TRIS/HCL PH 8, 8% PEG 8000 |
