4UD7
Structure of the stapled peptide YS-02 bound to MDM2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-07-03 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.148, 69.538, 78.464 |
Unit cell angles | 90.00, 102.36, 90.00 |
Refinement procedure
Resolution | 76.650 - 1.600 |
R-factor | 0.1405 |
Rwork | 0.138 |
R-free | 0.17838 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ycr |
RMSD bond length | 0.011 |
RMSD bond angle | 1.478 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.000 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.060 | 0.690 |
Number of reflections | 62522 | |
<I/σ(I)> | 12.1 | 1.9 |
Completeness [%] | 97.9 | 93.9 |
Redundancy | 3.7 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4 | 0.1 M SODIUM CITRATE PH 4.0 AND 15 % PEG8000 |