4U7B
Crystal structure of a pre-cleavage Mos1 transpososome
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 77 |
Detector technology | PIXEL |
Collection date | 2013-05-04 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.97625 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 96.471, 340.090, 160.360 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.750 - 3.090 |
R-factor | 0.2301 |
Rwork | 0.228 |
R-free | 0.26140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3hos |
RMSD bond length | 0.005 |
RMSD bond angle | 0.969 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 92.500 | 3.270 |
High resolution limit [Å] | 3.090 | 3.090 |
Rmerge | 0.134 | 0.442 |
Number of reflections | 69135 | |
<I/σ(I)> | 8.9 | 3.4 |
Completeness [%] | 99.7 | 99.7 |
Redundancy | 8 | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | 100 mM Ammonium acetate, 20 mM MgCl2, 50 mM Hepes pH 7.0, 5 % (w/v) PEG 8,000 |