4U6O

STRUCTURE OF THE CAP-BINDING DOMAIN OF INFLUENZA VIRUS POLYMERASE SUBUNIT PB2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-05-30
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	38.218, 64.377, 64.765
Unit cell angles	90.00, 91.04, 90.00

Refinement procedure

Resolution	38.210 - 1.300
R-factor	0.2099
Rwork	0.209
R-free	0.22690
RMSD bond length	0.005
RMSD bond angle	0.898
Data reduction software	HKL-2000
Data scaling software	HKL
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.320
High resolution limit [Å]	1.300	3.530	1.300
Rmerge	0.063	0.059	0.488
Total number of observations	285961
Number of reflections	74594
<I/σ(I)>	12
Completeness [%]	97.0	94.8	94.6
Redundancy	3.8	3.7	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	0.1mM Na-Malonate(pH7.0), 20% PEG 3350