4U62
Trichodysplasia spinulosa-associated polyomavirus (TSPyV) VP1 in complex with 3'-sialyllactose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-07-03 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 66.240, 152.840, 147.240 |
Unit cell angles | 90.00, 92.34, 90.00 |
Refinement procedure
Resolution | 40.000 - 1.550 |
R-factor | 0.15654 |
Rwork | 0.155 |
R-free | 0.18028 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4u5z |
RMSD bond length | 0.009 |
RMSD bond angle | 1.356 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.590 |
High resolution limit [Å] | 1.550 | 1.550 |
Number of reflections | 412698 | |
<I/σ(I)> | 13.4 | 2.2 |
Completeness [%] | 97.8 | 93.5 |
Redundancy | 3.8 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM Na-malonate pH 5.0, 10% PEG 3350 |