4TZX
Vibrio Vulnificus Adenine Riboswitch variant, grown in Mg2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-01-19 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 49.641, 152.839, 24.991 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.210 - 2.010 |
R-factor | 0.2272 |
Rwork | 0.224 |
R-free | 0.25660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1y26 |
RMSD bond length | 0.001 |
RMSD bond angle | 0.349 |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.210 | 2.130 |
High resolution limit [Å] | 2.010 | 2.010 |
Rmerge | 0.065 | 0.993 |
Number of reflections | 13466 | |
<I/σ(I)> | 21.1 | 2.1 |
Completeness [%] | 99.6 | 99.3 |
Redundancy | 5.7 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | Mg, PEG, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |