4TYI
Structural analysis of the human Fibroblast Growth Factor Receptor 4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-05-05 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.00000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 65.900, 65.900, 182.262 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 61.970 - 3.400 |
R-factor | 0.28146 |
Rwork | 0.277 |
R-free | 0.33994 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.025 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 61.970 | 3.690 |
High resolution limit [Å] | 3.400 | 3.400 |
Rmerge | 0.087 | 0.831 |
Number of reflections | 5806 | |
<I/σ(I)> | 0.104 | |
Completeness [%] | 96.4 | 96.8 |
Redundancy | 2.6 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 0.1 M Citric acid pH 4.0 1.6 M Ammonium sulfate final pH: 5 |