4TW2

Crystal Structure of SCARB2 in Neural Condition (pH7.5)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-13
Detector	RIGAKU SATURN A200
Wavelength(s)	0.91998
Spacegroup name	P 1 21 1
Unit cell lengths	57.796, 100.317, 105.173
Unit cell angles	90.00, 98.69, 90.00

Refinement procedure

Resolution	47.154 - 2.889
R-factor	0.2463
Rwork	0.244
R-free	0.29350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4f7b
RMSD bond length	0.007
RMSD bond angle	1.369
Data reduction software	SCALEPACK
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.900
High resolution limit [Å]	2.800	6.030	2.800
Rmerge	0.105	0.059	0.535
Number of reflections	28574
<I/σ(I)>	11.6
Completeness [%]	99.1	98.6	93.1
Redundancy	3.7	3.7	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	0.1 mol/L HEPES (pH 7.5), 10% v/v 2-Propanol, 20% w/v Polyethylene glycol 4000