4TUM
Crystal structure of Ankyrin Repeat Domain of AKR2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 103 |
Detector technology | CCD |
Collection date | 2010-06-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 68.331, 59.381, 84.860 |
Unit cell angles | 90.00, 107.33, 90.00 |
Refinement procedure
Resolution | 19.977 - 2.300 |
R-factor | 0.2074 |
Rwork | 0.206 |
R-free | 0.22410 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.398 |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.300 |
Number of reflections | 33241 |
<I/σ(I)> | 27.4 |
Completeness [%] | 98.7 |
Redundancy | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | PEG |