4TUE

Crystal structure of ASL-SufJ bound to Codon ACC-U on the Ribosome

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-22
Detector	RAYONIX MX300HS
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	211.282, 448.357, 616.604
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.802 - 3.500
R-factor	0.2156
Rwork	0.214
R-free	0.24990
RMSD bond length	0.006
RMSD bond angle	0.923
Data scaling software	XSCALE
Refinement software	PHENIX ((phenix.refine: 1.8.3_1479))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		3.600
High resolution limit [Å]	3.500	35.000	3.500
Rmerge	0.231	0.103	1.147
Rmeas	0.254	0.122	1.285
Total number of observations	4247078
Number of reflections	732004	445	58239
<I/σ(I)>	5.69	3.69	1.19
Completeness [%]	99.7	51.3	98.6
Redundancy	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl