4TT8
Crystal structure of the hydrolase domain of 10-formyltetrahydrofolate dehydrogenase (wild-type) complex with 10-formyl-5,8-dideazafolate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13C1 |
Synchrotron site | NSRRC |
Beamline | BL13C1 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2011-05-06 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 104.075, 52.694, 60.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.347 - 2.301 |
R-factor | 0.1819 |
Rwork | 0.179 |
R-free | 0.22400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ts4 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.968 |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.300 |
Number of reflections | 15370 |
<I/σ(I)> | 15.8 |
Completeness [%] | 100.0 |
Redundancy | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | Bis-Tris (0.1~0.2 M, pH 5.5) and PEG3350 (25~29%, w/v) |