4TQ3
Structure of a UbiA homolog from Archaeoglobus fulgidus bound to GPP and Mg2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-08 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97920 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.605, 218.922, 65.292 |
Unit cell angles | 90.00, 90.13, 90.00 |
Refinement procedure
Resolution | 30.338 - 2.408 |
R-factor | 0.223 |
Rwork | 0.221 |
R-free | 0.25530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4tq5 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.190 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 100.000 | 2.440 |
High resolution limit [Å] | 2.400 | 6.510 | 2.400 |
Rmerge | 0.101 | 0.062 | 0.488 |
Total number of observations | 182679 | ||
Number of reflections | 54488 | ||
<I/σ(I)> | 8.8 | ||
Completeness [%] | 92.6 | 98.7 | 91.7 |
Redundancy | 3.4 | 3.8 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 298 | 34% PEG 400, 100 mM Tris-HCL, 100 mM NaCl, 100 mM MgCl2, 1-oleoyl-rac-glycerol |