4TO4
Structure basis of cellular dNTP regulation, SAMHD1-GTP-dGTP-dCTP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.712, 145.843, 98.654 |
| Unit cell angles | 90.00, 114.75, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.198 |
| Rwork | 0.197 |
| R-free | 0.22530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bzb |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.617 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 5.700 | 2.100 |
| Rmerge | 0.122 | 0.086 | 0.839 |
| Total number of observations | 475194 | ||
| Number of reflections | 128708 | ||
| <I/σ(I)> | 9.7 | 1 | |
| Completeness [%] | 99.9 | 98.9 | 100 |
| Redundancy | 3.7 | 3.7 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 298 | SPG buffer, PEG 1500 |
