4TME
Crystal Structure of EutL from Clostridium Perfringens bound to ethanolamine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-04-24 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9795 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 87.960, 87.960, 251.910 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 83.043 - 1.700 |
R-factor | 0.1478 |
Rwork | 0.147 |
R-free | 0.16230 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.240 |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: dev_1525)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 83.043 | 1.740 | |
High resolution limit [Å] | 1.700 | 7.600 | 1.700 |
Rmerge | 0.088 | 0.040 | 1.240 |
Rmeas | 0.092 | 0.042 | 1.322 |
Total number of observations | 1370706 | ||
Number of reflections | 109437 | 1433 | 7950 |
<I/σ(I)> | 17.1 | 41.84 | 1.79 |
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 12.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.1M HEPES buffer, 5% PEG 8000, 8% ethylene glycol, crystals were soaked in 20mM ethanolamine |